Journal:

Article Title: Retinal Vascular Endothelial Growth Factor Induces Intercellular Adhesion Molecule-1 and Endothelial Nitric Oxide Synthase Expression and Initiates Early Diabetic Retinal Leukocyte Adhesion in Vivo

doi:

Figure Lengend Snippet: Flow Cytometric Analysis of Integrin Molecule Expression on Neutrophils

Article Snippet: The fluorescein isothiocyanate-conjugated mAbs LFA-1α chain (anti-rat CD11a), WT.5 (anti-rat CD11b), WT.3 (anti-rat CD18), and phycoerythrin-conjugated mAb OX-1 (anti-rat CD45) were all obtained from Pharmingen (San Diego, CA).

Techniques: Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: VASH-1 Regulates Oxidative Stress and Fibrosis in Diabetic Kidney Disease via SIRT1/HIF1α and TGFβ1/Smad3 Signaling Pathways

doi: 10.3389/fmolb.2020.00137

Figure Lengend Snippet: Western Blot Antibody information.

Article Snippet: , Anti-rat HIF1α , Rabbit , 132 , sc-10790 , 1:500 , Santa Cruz.

Techniques: Western Blot, Molecular Weight

Journal: Frontiers in Molecular Biosciences

Article Title: VASH-1 Regulates Oxidative Stress and Fibrosis in Diabetic Kidney Disease via SIRT1/HIF1α and TGFβ1/Smad3 Signaling Pathways

doi: 10.3389/fmolb.2020.00137

Figure Lengend Snippet: Effects on pathway of Vash1 siRNA transfection in high glucose cultured RMCs. Relative expression levels of (A) Vash1 , (B) Sirt1 , (C) Hif1a , (D) Tgfb1 , and (E) Smad3 mRNA, (F) Western blot protein bands, relative expression levels of (G) SIRT1, (H) HIF1α, (I) TGFβ1, (J) pSmad3/Smad3. *Compared with the NG group, P < 0.05; # Compared with the HG group, P < 0.05. Data were expressed as mean ± SD, and results were repeated three times.

Article Snippet: , Anti-rat HIF1α , Rabbit , 132 , sc-10790 , 1:500 , Santa Cruz.

Techniques: Transfection, Cell Culture, Expressing, Western Blot

Journal: Frontiers in Molecular Biosciences

Article Title: VASH-1 Regulates Oxidative Stress and Fibrosis in Diabetic Kidney Disease via SIRT1/HIF1α and TGFβ1/Smad3 Signaling Pathways

doi: 10.3389/fmolb.2020.00137

Figure Lengend Snippet: Effect of Sirt1, Hif1a siRNA, and the TGFβ1/Smad3 pathway inhibitor on pathway expression in high glucose-cultured RMCs. Relative expression levels of (A) Vash1 , (B) Sirt1 , (C) Hif1a , (D) Tgfb1 , and (E) Smad3 mRNA, (F) Western blot protein bands, relative expression levels of (G) SIRT1, (H) HIF1α, (I) TGFβ1, (J) pSmad3/Smad3. *Compared with the HG group, P < 0.05. Data were expressed as mean ± SD, and results were repeated three times.

Article Snippet: , Anti-rat HIF1α , Rabbit , 132 , sc-10790 , 1:500 , Santa Cruz.

Techniques: Expressing, Cell Culture, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Protection of Male Rat Offspring against Hypertension Programmed by Prenatal Dexamethasone Administration and Postnatal High-Fat Diet with the Nrf2 Activator Dimethyl Fumarate during Pregnancy

doi: 10.3390/ijms20163957

Figure Lengend Snippet: Protein levels of nutrient-sensing signals at 16-week-old offspring kidney exposed to prenatal dexamethasone (DEX), postnatal high-fat diet (HF), and dimethyl fumarate (DMF). ( A ) Presentative western blots of SIRT1 (120 kDa), phosphorylated AMPKα2 (62 kDa), and PGC-1α (90 kDa). Relative abundance of renal cortical ( B ) SIRT1, ( C ) p-mTOR, and ( D ) PGC-1α as quantified. n = 8/group. * p < 0.05 vs. control; # p < 0.05 vs. DEX; † p < 0.05 vs. HF; ‡ p < 0.05 DEX+HF.

Article Snippet: We used the following primary antibodies: a rabbit polyclonal anti-rat SIRT1 antibody (1:1000, overnight incubation; Abcam, Cambridge, MA, USA), a rabbit polyclonal anti-rat phosphorylated AMPKα2 antibody (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and a rabbit polyclonal anti-rat PGC-1α antibody (1:1000, overnight incubation; Santa Cruz Biotechnology).

Techniques: Western Blot, Control