Journal:

Article Title: Importance of Macrophage Inflammatory Protein-1? and Splenic Macrophages in Neurodegeneration Induced by PVC-211 Murine Leukemia Virus

doi: 10.1016/j.virol.2010.10.012

Figure Lengend Snippet: PVC-211 MuLV-infected rats were treated with anti-MIP-1α antibodies beginning on the day prior to virus injection and continuing for 8 more injections until 17 days post virus injection. Control PVC-211 MuLV-infected rats were treated with non-immune serum or water. A total of 19 anti- MIP-1α-treated rats and 18 control rats from 4 different experiments were followed. A. Percentage of PVC-211 MuLV-infected rats with complete paralysis at various time points after virus injection. ▲: PVC-211 MuLV-infected rats given anti-MIP-1α antibodies; ○: PVC-211 MuLV-infected control rats. B. PVC-211 MuLV gp70 envelope expression in spleen, brainstem and serum from anti-MIP-1α-treated, control PVC-211 MuLV-infected or uninfected rats at 24 dpi or at the end the observation period (40 dpi) (asterisk). β-actin was used as a loading control. The amount of MuLV gp70 was quantitated in relationship to the loading control and is represented as a gp70/actin ratio at the bottom of each lane.

Article Snippet: The administration of rabbit anti-rat MIP-1α antibody (PeproTech Inc., Rocky Hill, NJ; Cat. # 500-P77) was started at birth [1 day before virus injection (−1 dpi)] and continued on days 0, 3, 5, 7, 10, 12, 14 and 17 dpi.

Techniques: Infection, Virus, Injection, Control, Expressing

Journal:

Article Title: Importance of Macrophage Inflammatory Protein-1? and Splenic Macrophages in Neurodegeneration Induced by PVC-211 Murine Leukemia Virus

doi: 10.1016/j.virol.2010.10.012

Figure Lengend Snippet: A: Protein levels of MIP-1α were detected by a specific immunoassay using serum from PVC-211 MuLV-infected rats collected at different time points after virus injection. Gray bars: rats given anti-MIP-1α; Black bars: control rats. Data represent means ± SEM. P<0.05 at 14 dpi; P> 0.05 at 7 and 21 dpi. B: Activated microglia (upper panels) were detected at 21 dpi in frozen sections of brainstem from PVC-211 MuLV-infected rats by immunofluorescent (IF) staining with ED1 antibody (indicated by green color). Left: Control PVC-211 MuLV-infected rats; Middle and Right: PVC-211 MuLV-infected rats treated with anti-MIP-1α antibodies. 20X. Comparison of the average number of activated microglia (as determined by counting the number of ED1 antibody-positive cells per 20X field) in the brainstem of 3 control rats and 3 anti-MIP-1α antibody-treated rats is shown at the bottom. Equivalent numbers of cells (as determined by DAPI staining; not shown) were examined in each case.

Article Snippet: The administration of rabbit anti-rat MIP-1α antibody (PeproTech Inc., Rocky Hill, NJ; Cat. # 500-P77) was started at birth [1 day before virus injection (−1 dpi)] and continued on days 0, 3, 5, 7, 10, 12, 14 and 17 dpi.

Techniques: Infection, Virus, Injection, Control, Staining, Comparison

Journal:

Article Title: Importance of Macrophage Inflammatory Protein-1? and Splenic Macrophages in Neurodegeneration Induced by PVC-211 Murine Leukemia Virus

doi: 10.1016/j.virol.2010.10.012

Figure Lengend Snippet: A: The protein levels of MIP-1α were detected by a specific immunoassay using serum from PVC-211 MuLV-infected rats collected at different time points after virus injection. Gray bars: splenectomized rats; Black bars: control rats. Data represent means ± SEM. P<0.05 at 14 dpi; P> 0.05 at 9 and 24 dpi. B: Activated microglia (upper panels) were detected at 21 dpi in frozen sections of the brainstems of non-splenectomized (left panel) or splenectomized (right and middle panel) PVC-211 MuLV-infected rats by immunofluorescent (IF) staining with ED1 antibody (indicated by green color). 20X. Comparison of the average number of activated microglia (as determined by counting the number of ED1 antibody positive cells per 20X field) in the brainstem of 3 control rats and 3 splenectomized rats is shown at the bottom. Equivalent numbers of cells (as determined by DAPI staining; not shown) were examined in each case.

Article Snippet: The administration of rabbit anti-rat MIP-1α antibody (PeproTech Inc., Rocky Hill, NJ; Cat. # 500-P77) was started at birth [1 day before virus injection (−1 dpi)] and continued on days 0, 3, 5, 7, 10, 12, 14 and 17 dpi.

Techniques: Infection, Virus, Injection, Control, Staining, Comparison

Journal:

Article Title: Retinal Vascular Endothelial Growth Factor Induces Intercellular Adhesion Molecule-1 and Endothelial Nitric Oxide Synthase Expression and Initiates Early Diabetic Retinal Leukocyte Adhesion in Vivo

doi:

Figure Lengend Snippet: Flow Cytometric Analysis of Integrin Molecule Expression on Neutrophils

Article Snippet: The fluorescein isothiocyanate-conjugated mAbs LFA-1α chain (anti-rat CD11a), WT.5 (anti-rat CD11b), WT.3 (anti-rat CD18), and phycoerythrin-conjugated mAb OX-1 (anti-rat CD45) were all obtained from Pharmingen (San Diego, CA).

Techniques: Expressing